Anti-IL-4 Antibody [CC313]

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Descriere

Specificații

Brand

Antibodies

Mărime

500µg

Țintă

IL-4

Applications

ELISA

Reactivity

Bovine, Sheep, Goat

Formulation

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein A affinity chromatography of tissue culture supernatant.

Isotype

IgG2a

Recommended dilutions

ELISA: 2 µg/ml - 5 µg/ml

Clonă

CC313

Imunogen

Recombinant bovine IL-4.

Specificitate

This antibody recognises bovine interleukin 4 (IL-4), also known as B-cell stimulatory factor 1. Bovine interleukin-4 is a 15 kDa, 135 amino acid peptide which is processed to a mature secreted form with the cleavage of a 24 amino acid, N-terminal signal peptide resulting in a 12.6 kDa active cytokine, the apparent MW may be altered by glycosylation. IL-4 is expressed by a range of cells including T and B cells, macrophages and monocytes. It plays an important role in the regulation of bovine T and B cell responses. Mouse anti Bovine interleukin-4, clone CC313 has been used successfully as a coating antibody in a sandwich ELISA with biotinylated Mouse anti Bovine IL-4 antibody, clone CC314 (MCA2372B) as a detection reagent both with bovine and ovine samples. Clones CC313 and CC314 have been used in the reverse configuration for the measurement of interleukin-4 in caprine samples.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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