Anti-IL-4 Antibody - BSA and Azide free

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

IL-4

Applications

WB, ELISA, Functional Assays

Reactivity

Mouse

Formulation

Supplied in Phosphate Buffered Saline.

Conjugate

Unconjugated

Host

Rabbit

Clonality

Polyclonal

Product form

Lyophilized

Concentration

1 mg/ml after reconstitution.

Isotype

IgG

Recommended dilutions

ELISA: 0.5 µg/ml, Functional Assays: 0.25 µg/ml - 0.40 µg/ml, WB: 0.1 µg/ml - 0.2 µg/ml

Reconstitution

Reconstitute with 0.1 ml of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended..NB: For functional studies do not add sodium azide.

Imunogen

Recombinant mouse IL-4.

Specificitate

This antibody recognises mouse interleukin-4 (IL-4), also known as B-cell stimulatory factor 1, a 120 amino acid pleiotropic cytokine cleaved from a 140 amino acid precursor which interacts with multiple cell lineages, including T cells, fibroblasts and macrophages. Interleukin-4 is one of the most important factors involved in B cell IgG1 and IgE class switching. IL-4 acts as a regulator of low affinity Fc receptor for IgE (CD23) expression on both lymphocytes and monocytes (Miyajima et al. 1991, Hudak et al. 1987). IL-4 acts through binding to it's cognate receptor CD124, a receptor which also binds the related cytokine IL-13.Interleukin -4 plays an important role in the alternative activation of murine macrophages as indicated by the ability to potently up-regulate the expression and activity of CD206, the macrophage mannose receptor.

Storage

Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Documentație

• Fișă tehnică (PDF)se deschide pe site-ul producătorului