Anti-HLA B27 Antibody [HLA-ABC-m3] (FITC)

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Descriere

Specificații

Brand

Antibodies

Mărime

100T

Țintă

HLA-B27

Applications

Flow Cytometry

Reactivity

Human

Formulation

Supplied in Phosphate Buffered Saline with 0.2% BSA and <0.1% Sodium Azide.

Conjugate

FITC

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

50 µg/ml

Purification

Protein A affinity chromatography of ascites.

Isotype

IgG2a

Recommended dilutions

Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 10⁶ cells or 100µl of whole blood.

Clonă

HLA-ABC-m3

Imunogen

Immune complex precipitated from an HLA-B27 positive cell line by anti HLA antibody and staphylococcal protein A.

Specificitate

This antibody recognises the HLA-B27 alloantigen and reacts with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA-B27 + and precipitates cell surface molecules of 43 and 12 kDa, corresponding to the HLA Class 1 heavy chain and beta 2 microglobulin.Scatchard affinity analysis shows that Mouse anti Human HLA B27 antibody, clone HLA-ABC-m3 has a higher affinity for HLA-B27 (9.7 x 108M-1) than for HLA B7 (9.5 x 107 M-1).Flow cytometry - subjects expected fluorescence intensity:Heterozygous HLA-B27+ = Strong.Homozygous HLA-B27+ = Strong.Heterozygous HLA-B7+ = Faint.Non B27, Non B7 = Negative.

Storage

Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Documentație

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