Anti-Histone H3 (di methyl Lys9) Antibody

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

Histone H3

Applications

ChIP, IF, WB

Reactivity

Human, Broad

Formulation

Supplied in Phosphate Buffered Saline with 0.035% Sodium Azide and 30% Glycerol.

Conjugate

Unconjugated

Host

Rabbit

Clonality

Polyclonal

Product form

Liquid

Concentration

Lot Specific

Isotype

IgG

Recommended dilutions

ChIP: 10 µg per ChIP, WB: 0.5 µg/ml - 1 µg/ml, IF: 1 µg/ml, WB: 1 µg/ml - 2 µg/ml

Imunogen

A peptide including di-methylated lysine 9 of histone H3.

Specificitate

This antibody recognises Histone H3 when di-methylated at lysine 9.Histone H3 is one of the four core histones that make up the nucleosome core particle. Histone H3 is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). Histones can be mono-, di- or tri-methylated by histone methyltransferases. Depending on which amino acid residues are methylated, methylation of histones may increase or decrease the transcription of genes. Methylation events that weaken the binding between histone tails and DNA lead to increased transcription because they make the DNA more accessible to transcription factor proteins and RNA polymerase. Methylation of histones is therefore crucial for the regulation of gene expression. Lysine 9 of histone H3 may be mono-, di- or tri-methylated by SuvH39H1 or G9a, usually leading to transcriptional repression. Wide species cross-reactivity is expected from Rabbit anti Human di-methyl-histone H3 (Lys9) antibody based on sequence.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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