Anti-Histone H2B (mono methyl Lys5) Antibody

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Descriere

Specificații

Brand

Antibodies

Mărime

100µl

Țintă

Histone H2B

Applications

WB

Reactivity

Human, Broad

Formulation

Supplied in Phosphate Buffered Saline with 0.035% Sodium Azide and 30% Glycerol.

Conjugate

Unconjugated

Host

Rabbit

Clonality

Polyclonal

Product form

Liquid

Concentration

Lot Specific

Isotype

IgG

Recommended dilutions

WB: 1:500-1:1,000

Imunogen

Synthetic peptide of mono-methyl lysine 5 of human histone H2B.

Specificitate

This antibody recognises Histone H2B when mono-methylated at lysine 5.Histone H2B is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). The presence of post-translational modifications and specialized variants of histone H2B helps the regulation of chromatin structure and function. It has been suggested that changes in methylation of histone H2B may be involved in heat-shock mechanisms. Mono-methylation of lysine 5 is thought to occur at active promoters downstream of the transcription start sight. Wide species cross-reactivity is expected from Rabbit anti Human mono-methyl-histone H2B (Lys5) antibody based on sequence.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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