Anti-Histone H2B (acetyl Lys 15) Antibody

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Descriere

Specificații

Brand

Antibodies

Mărime

100µl

Țintă

Histone H2B

Applications

WB

Reactivity

Human

Formulation

Supplied in Phosphate Buffered Saline with 0.035% Sodium Azide and 30% Glycerol.

Conjugate

Unconjugated

Host

Rabbit

Clonality

Polyclonal

Product form

Liquid

Concentration

Lot Specific

Isotype

IgG

Recommended dilutions

WB: 1:500-1:1,000

Imunogen

A peptide containing acetylated lysine 15 of human histone H2B.

Specificitate

This antibody recognises Histone H2B when acetylated at lysine 15.Histone H2B is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). Histone H2B aids the regulation of chromatin structure and function via post-translational modifications of specialized histone variants. Lysine residues within the N-terminal tail protruding from the histone core of the nucleosome are acetylated and deacetylated as part of gene regulation. Hyperacetylation of histone tails makes chromatin more accessible to DNA-binding proteins by weakening histone-DNA and nucleosome-nucleosome interactions. Acetylation of a specific lysine residues on histones allows binding to bromine-containing domains of particular transcription and chromatin regulatory proteins. Wide species cross-reactivity is expected from Rabbit anti Human histone H2B (Ac15) antibody based on sequence.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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