Anti-GAPDH Antibody

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

GAPDH

Applications

WB, IHC-P, ELISA

Reactivity

Human, Dog, Mouse, Pig, Rat

Formulation

Supplied in TRIS Buffered Saline, 0.5% BSA and 0.02% Sodium Azide.

Conjugate

Unconjugated

Host

Goat

Clonality

Polyclonal

Product form

Liquid

Concentration

500 µg/ml

Isotype

IgG

Recommended dilutions

ELISA: 1:2,000, IHC-P: 0.3 µg/ml, WB: 0.01 - 0.03 µg/ml

Molecular weight

Approximately 35 kDa in human tonsil, mouse spleen and HeLa cell lysates.

Secvență

C-HQVVSSDFNSDT

Imunogen

A peptide sequence of C-HQVVSSDFNSDT corresponding to the C-terminus of GAPDH (NP_002037.2).

Specificitate

This antibody recognises an epitope within the C-terminal (CT) region of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a ~36 kDa multifunctional protein whose main function is to catalyse the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate, in conjunction with inorganic phosphate and nicotinamide adenine dinucleotide (NAD). This reaction is an important energy yielding step in carbohydrate metabolism. GAPDH translocates to the nucleus under a variety of stressors, most of which are associated with oxidative stress, whereby it mediates cell death. GAPDH binds to several proteins that are responsible for neurodegenerative diseases, such as amyloid precursor protein and Huntingtin.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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