Anti-Cortisol Aptamer [CSS.1] (Biotin)

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Descriere

Specificații

Brand

Antibodies

Mărime

25µg

Țintă

Cortisol

Formulation

Lyophilized from nuclease-free water (no residual salts).

Conjugate

Biotin

Product form

Lyophilized

Concentration

Reconstitution dependent.

Purification

HPLC

Recommended dilutions

Small Organic Molecule

Molecular weight

12,881.4 Da

Purity

Affinity (Kd) = 245 nM

Protein Length

42 nt

Extinction coefficient

395100 M⁻¹cm⁻¹

Clonă

CSS.1

Secvență

5'-dGpdApdCpdGpdApdCpdGpdCpdCpdCpdGpdCpdApdTpdGpdTpdTpdCpdCpdApdTpdGpdGpdApdTpdApdGpdTpdCpdTpdTpdGpdApdCpdTpdApdGpdTpdCpdGpdTpdCp-3'

Imunogen

Selection Buffer 1 (CSS.1 aptamer): 20mM HEPES Buffer, pH 7.5, 5mM Potassium Chloride, 1M Sodium Chloride, and 10mM Magnesium Chloride. Selection Buffer 1-1: 20mM HEPES Buffer, pH 7.5, 5mM Potassium Chloride, 100mM Sodium Chloride, and 10mM Magnesium Chloride. Selection Buffer 1-2: 20mM HEPES Buffer, pH 7.5, 5mM Potassium Chloride, 100mM Sodium Chloride, and 2mM Magnesium Chloride. Selection Buffer 1-3: 20mM HEPES Buffer, pH 7.5, 5mM Potassium Chloride, and 100mM Sodium Chloride. Selection Buffer 2 (15-1 aptamer): 50mM HEPES Buffer, pH 7.5, with 137mM Sodium Chloride and 5mM Magnesium Chloride. Selection Buffer 3 (avoid adsorption of HEPES Buffer on AuNPs): pH 7.5,10mM Phosphate, and 1mM Magnesium Chloride. Artificial interstitial fluids (ISF): 10mM HEPES Buffer, pH 7.5, with 2.5mM Calcium Chloride, 3.5mM Potassium Chloride, 0.7mM Magnesium Sulfate, 123mM Sodium Chloride, 1.5mM Potassium Phosphate, and 7.4mM Sucrose. Binding Temperature: 25°C.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.

Documentație

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