Rabbit Cortisol ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

Cortisol

Applications

Competitive ELISA

Reactivity

Rabbit

Recommended dilutions

Estimate the expected concentration of samples prior to the assay. Serum and plasma samples are recommended for use undiluted whenever possible. If sample dilution is required, use the sample diluent provided in the kit to maintain assay consistency.

Assay type

Competitive (quantitative)

Sensitivity

2.87 ng/ml

Sample type

Serum and plasma

Cross reactivity

Specific for Cortisol with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

6.25-400 ng/ml

Assay Time

1h 30m

Principiul testului

Rabbit Cortisol ELISA Kit (A327159) employs a rapid competitive Enzyme-Linked Immunosorbent Assay (ELISA) technique for the quantitative measurement of rabbit Cortisol in serum and plasma. Antigen specific for Cortisol has been pre-coated onto a 96-well microtiter plate. Standards and test samples are added to the wells together with HRP-conjugated detection reagent working solution. During incubation, Cortisol present in the sample competes with the immobilized antigen for binding to the HRP-conjugated detection reagent. Following incubation, unbound components are removed by washing. TMB substrate solution is then added to visualize the HRP enzymatic reaction, producing a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is inversely proportional to the concentration of Cortisol present in the sample. The concentration of Cortisol is determined by measuring the O.D. absorbance at 450 nm using a microplate reader and comparing the values to the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C as instructed in the Protocol Manual for up to 6 months. Do not use past expiration date!