Anti-CD45 Antibody [IBL-3/16]

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

250µg

Țintă

CD45

Applications

IP, Flow Cytometry, IHC-Fr, IF

Reactivity

Mouse

Formulation

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Conjugate

Unconjugated

Host

Rat

Clonality

Monoclonal

Product form

Liquid

Concentration

500 µg/ml

Purification

Protein G affinity chromatography of tissue culture supernatant.

Isotype

IgG1

Recommended dilutions

Flow Cytometry: 1:50 - 1:100, IHC-Fr 1: 1:50 - 1:100, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Cross reactivity

This antibody does not cross react with Rat, Human, or Chicken

Clonă

IBL-3/16

Imunogen

Purified B cells from mouse lymph nodes.

Specificitate

This antibody recognises mouse Receptor-type tyrosine-protein phosphatase C, also known as CD45, Leukocyte common antigen, T200 or Lymphocyte antigen 5 (Ly5). CD45 is a 1291 amino acid ~175kDa single pass type I transmembrane glycoprotein belonging to the protein -tyrosing phosphatase family. CD45 has two fibronectin type-III domains and two tyrosine protein phosphatase domains (UniProt:: P06800).Multiple isoforms are generated by alternative splicing with isoforms having differing deletions in the N-terminal region. Rat anti Mouse CD45 antibody, clone IBL-3/16 is expected to recognize all isoforms of murine CD45.Rat anti Mouse CD45 antibody, clone IBL-3/16 has been used successfully for the identification of CD45 in murine samples using Immunohistochemical (both cryo and FFPE), immunofluorescence and western blotting techniques (Kondo et al. 2011, Cuadros et al. 2006).

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

• Fișă tehnică (PDF)se deschide pe site-ul producătorului