Anti-CD4 Antibody [RPA-T4] (PE)

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Descriere

Specificații

Brand

Antibodies

Mărime

100T

Țintă

CD4

Applications

Flow Cytometry

Reactivity

Human

Formulation

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Conjugate

PE

Host

Mouse

Clonality

Monoclonal

Product form

Lyophilized

Concentration

Reconstitution dependent.

Purification

Protein G affinity chromatography of tissue culture supernatant.

Isotype

IgG1

Recommended dilutions

Flow Cytometry: Neat - 1:2, Use 10µl of the suggested working dilution to label 10⁶ cells or 100µl whole blood.

Reconstitution

Reconstitute with 1 ml of distilled water.

Clonă

RPA-T4

Imunogen

Human PHA blasts.

Specificitate

This antibody recognises human CD4, a ~55 kDa cell surface glycoprotein, primarily expressed on a subpopulation of T lymphocytes, on peripheral blood monocytes and on tissue macrophages. Epitope mapping shows that antibodies, produced by clone RPA-T4, recognize an epitope within domain 1 of the extracellular region of the CD4 molecule. Mouse anti human CD4 antibody, clone RPA-T4 blocks gp120-CD4 interaction and inhibits syncytium formation (Piatier-Tonneau et al, 1997). The use of Mouse anti Human CD4:Low Endotoxin (MCA1267EL) is recommended for functional assays.

Storage

Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Documentație

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