Anti-CD4 Antibody [RM4-5] (FITC)

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

CD4

Applications

Flow Cytometry

Reactivity

Mouse

Formulation

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Conjugate

FITC

Host

Rat

Clonality

Monoclonal

Product form

Liquid

Concentration

100 µg/ml

Purification

Protein G affinity chromatography of tissue culture supernatant.

Isotype

IgG2a

Recommended dilutions

Flow Cytometry: Neat - 1:5, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).

Clonă

RM4-5

Imunogen

BALB/c mouse thymocytes.

Specificitate

This antibody recognises mouse CD4, a 55 kDa protein also known as Ly-4 and L3T4. CD4 is a single chain transmembraneous glycoprotein which belongs to the immunoglobulin superfamily, and is primarily expressed on peripheral blood monocytes and tissue macrophages. CD4 is also expressed on a subpopulation of regulatory T cells (CD4+CD25+), which play a key role in the maintenance of self tolerance.Rat anti Mouse CD4 antibody, clone RM4-5 can be used for in vitro blocking of ligand binding, as well as in vitro CD4+ T cell depletions.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Documentație

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