Anti-CD34 Antibody [SPM123]

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

CD34

Applications

Flow Cytometry, IF, IHC-P

Reactivity

Human, Cynomolgus Monkey, Rhesus Monkey

Formulation

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

200 µg/ml

Purification

Protein A/G chromatography.

Isotype

IgG1

Recommended dilutions

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, IHC-P: 1-2 µg/ml

Molecular weight

~ 90, 110 kDa

Cross reactivity

This antibody does not cross react with Rat, Sheep, Bovine, or Canine.

Light chain

kappa

Clonă

SPM123

Imunogen

Detergent solubilized vesicular suspension prepared from human term placenta.

Specificitate

This antibody recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. On the basis of differential sensitivity to degradation by specific enzymes, epitopes of monoclonal antibodies to CD34 are classified intothree main categories, class I, class II and class III. It is a class II antibody whose epitope is resistant to neuraminidase but sensitive to glycoprotease and chymopapain.CD34 expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, CD34 is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. Anti-CD34 labels 85% of angiosarcoma and Kaposis sarcoma, but with a lower specificity.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Documentație

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