Anti-CD34 Antibody [ICO-115] (CF® 488)

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Descriere

Specificații

Brand

Antibodies

Mărime

100T

Țintă

CD34

Applications

Flow Cytometry, IF

Reactivity

Human, Rat

Formulation

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Conjugate

CF®488

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

100 µg/ml

Purification

Protein A/G chromatography.

Isotype

IgG1

Recommended dilutions

Flow Cytometry: 5 µl / million cells or 5 µl / 100 µl whole blood, IF: 1:50-1:100

Molecular weight

~ 90, 110 kDa

Light chain

kappa

Clonă

ICO-115

Imunogen

Blast cells of a chronic myeloid leukemia patient.

Specificitate

This antibody recognizes a carbohydrate epitope on a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34 (VI international workshop on human differentiation antigens). Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels 85% of angiosarcoma and Kaposis sarcoma, but shows low specificity.

Storage

Store at 4-8°C. Antibody is stable for 24 months.

Documentație

• Fișă tehnică (PDF)se deschide pe site-ul producătorului