Anti-CD31 Antibody [TLD-3A12] - BSA and Azide free

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Descriere

Specificații

Brand

Antibodies

Mărime

500µg

Țintă

CD31

Applications

Flow Cytometry, WB, ELISA, IHC-Fr, IF

Reactivity

Rat, Rhesus Monkey, Pig

Formulation

Supplied in Phosphate Buffered Saline.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein G affinity chromatography of tissue culture supernatant.

Isotype

IgG1

Recommended dilutions

Flow Cytometry: 1:10 - 1:100, IHC-Fr: 1:10 - 1:100, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Clonă

TLD-3A12

Imunogen

Activated, Lewis rat derived microglial cells.

Specificitate

This antibody recognises rat PECAM-1 (CD31), a 661 amino acid type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leucocytes.Clone TLD-3A12 has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen (Stevenson, K.S. et al.2009). Mouse anti Rat CD31 antibody, clone TLD-3A12 is suitable for use in IHC on formalin-fixed paraffin-embedded sections pre-treated with 0.2M boric acid, pH7.0. Mouse anti Rat CD31, clone TLD-3A12 has been shown to be cross-reactive with endothelial cells derived from rhesus macaque

Storage

Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.

Documentație

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