Anti-CD31 Antibody [LCI-4] (PE)

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Descriere

Specificații

Brand

Antibodies

Mărimi disponibile

25T · 100T

Țintă

CD31

Applications

Flow Cytometry

Reactivity

Porcine, Human

Formulation

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Conjugate

PE

Host

Mouse

Clonality

Monoclonal

Product form

Lyophilized

Concentration

Reconstitution dependent.

Purification

Affinity chromatography of tissue culture supernatant.

Isotype

IgG1

Recommended dilutions

Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 10⁶ cells or 100µl whole blood

Cross reactivity

This antibody does not cross-react with Mouse.

Reconstitution

Pack Size of 100 Tests: Reconstitute with 1 ml of distilled water. Pack Size of 25 Tests: Reconstitute with 0.25 ml of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution.

Clonă

LCI-4

Imunogen

Porcine CD31/human IgGFc fusion protein.

Specificitate

This antibody recognises porcine CD31, also known as Platelet endothelial cell adhesion molecule (PECAM-1). CD31 is constitutively expressed by platelets, monocytes and some lymphocytes, it is expressed by endothelial cells at a level, an order of magnitude greater that of other cell types. The extracellular region contains six Ig-like domains. Mouse anti Pig CD31, clone LCI-4 is cross reactive with human CD31 and binds to the 5th extracellular Ig domain, proximal to the transmembrane region as demonstrated by human CD31 domain deletion mutants. Mouse anti Pig CD31, clone LCI-4 immunoprecipitates a protein of ~130 kDa from lysates of porcine aortic endothelial cells and is strongly expressed at cell junctions.

Storage

Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Documentație

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