Anti-CD14 Antibody [CC-G33]

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

CD14

Applications

Flow Cytometry, IF

Reactivity

Bovine, Sheep, Human, Water Buffalo

Formulation

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein A affinity chromatography of tissue culture supernatant.

Isotype

IgG1

Recommended dilutions

Flow Cytometry: 1:10 - 1:200, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl.

Clonă

CC-G33

Imunogen

Partially purified polypeptides isolated from bovine leucocyte cell surface membrane.

Specificitate

This antibody recognises bovine CD14.CD14 is a GPI-anchored membrane glycoprotein and monocyte/macrophage differentiation antigen, belonging to the lipopolysaccharide receptor family, also expressed weakly on microglia and Langerhans cells. CD14 acts as a receptor for the potent bacterial endotoxin, lipopolysaccharide (LPS), facilitated by LPS-binding protein (LBP). The binding of LPS to CD14 results in cell activation and the release of cytokines and the inflammatory response, and has been shown to upregulate the cell surface expression of adhesion molecules. Mouse anti Bovine CD14 clone CC-G33 cross-reacts with human CD14 expressed on transfected COS-7 cells , ovine CD14 and Water buffalo (Bubalus bubalis) CD14,.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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