Anti-CD14 Antibody [UCHM1] (APC)

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărimi disponibile

25T · 100T

Țintă

CD14

Applications

Flow Cytometry

Reactivity

Human, Cynomolgus monkey, Rhesus Monkey, Fish, Trout

Formulation

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Conjugate

APC

Host

Mouse

Clonality

Monoclonal

Product form

Lyophilized

Concentration

Reconstitution dependent.

Purification

Protein G affinity chromatography of tissue culture supernatant.

Isotype

IgG2a

Recommended dilutions

Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Reconstitution

Pack Size of 100 Tests: Reconstitute with 1 ml of distilled water. Pack Size of 25 Tests/0.25ml: Reconstitute with 0.25 ml of distilled water.

Clonă

UCHM1

Imunogen

Human Thymocytes followed by peripheral blood mononuclear cells.

Specificitate

This antibody recognises a cell surface antigen of ~55 kDa, known as CD14.The CD14 molecule is found predominantly on monocytes and macrophages in flow cytometry, it is less strongly expressed on granulocytes, and is absent from stem cells and myeloid cells of very early differentiation states. In immunohistology the CD14 molecule is found to be present on Langerhans cells, follicular dendritic cells, histiocytes and high endothelial venules. Antibodies to the CD14 molecule are known to induce oxidative burst formation. In tonsil tissue sections UCHM1 gives positive staining reactions with monocytic cells, the interfollicular tissue macrophages seen under the capsule, and dendritic reticulum cells. Skin Langerhans cells are always negative. UCHM1 also reacts with Kupffer cells and sinus lining cells on the liver.

Storage

Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Documentație

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