Anti-CD11a Antibody [BL1H8] (FITC)

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

CD11a

Applications

Flow Cytometry

Reactivity

Porcine, Sheep, Bovine

Formulation

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Conjugate

FITC

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

100 µg/ml

Purification

Protein G affinity chromatography of tissue culture supernatant.

Isotype

IgG2b

Recommended dilutions

Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Clonă

BL1H8

Imunogen

Porcine alveolar macrophages.

Specificitate

This antibody recognises porcine CD11a, binding to a conformational epitope on the alpha chain of LFA-1 (CD11a/CD18), expressed on a range of cells including peripheral blood lymphocytes, monocytes, granulocytes and alveolar macrophages. Clone BL1H8 immunoprecipitates two bands of 170 kDa (CD11a) and 95 kDa (CD18) from porcine alveolar lysates, pre-clearance of the lysates completely removed the 170 kDa band , strongly suggesting the epitope for this antibody is located on the CD11a chain, although some involvement of residues on the LFA-1 ß chain could not be formally ruled out (Alvarez et al. 2000 ). Mouse anti Pig CD11a, clone BL1H8 was clustered as porcine CD11a at the Third International Workshop on Swine Leukocyte Differentiation Antigens. Mouse anti pig CD11a, clone BL1H8 is reported to inhibit Con-A driven T-cell proliferation and the mixed lymphocyte reaction (Alvarez et al. 2000 ).

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Documentație

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