Anti-beta Actin Antibody

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

beta Actin

Applications

WB

Reactivity

Human, Mouse, Rat

Formulation

Supplied in Phosphate Buffered Saline with 0.2% Gelatin and 0.1% Sodium Azide.

Conjugate

Unconjugated

Host

Rabbit

Clonality

Polyclonal

Product form

Liquid

Concentration

200 µg/ml

Purification

Affinity-chromatography

Isotype

IgG

Recommended dilutions

WB: 1:200

Molecular weight

Aproximately 42 kDa when run under reducing conditions

Imunogen

A peptide corresponding to the N-terminus human actin beta.

Specificitate

This antibody recognises the product of the ACTB gene, cytoplasmic actin beta, also known as actin cytoplasmic and beta actin1. Actin beta is ubiquitously expressed in all eukaryotic cells, playing a critical role in cell survival, motility and re-modeling.Actin beta is highly conserved throughout evolution, with all vertebrate and invertebrate species reviewed to date bearing a 98% or greater amino acid primary sequence identity to human actin beta. Plant species also exhibit a high degree of primary sequence conservation displaying considerable similarity to animal actin beta. For example, nearly 90% sequence identity is observed between human ACTB and the model plant species Arabidopsis thaliana (mouse eared cress) ACT1 (Arabidopsis) gene products. Actin beta is a commonly used western blotting loading control.

Storage

Store undiluted at 4°C. Do not freeze! Should this product contain a precipitate we recommend microcentrifugation before use.

Documentație

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