Anti-beta Actin Antibody

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

beta Actin

Applications

WB, IHC-Fr

Reactivity

Human, Mouse, Rat

Formulation

Supplied in phosphate-buffered saline with 0.02% Sodium Azide.

Conjugate

Unconjugated

Host

Rabbit

Clonality

Polyclonal

Product form

Liquid

Concentration

1 mg/ml

Isotype

IgG

Recommended dilutions

IHC-Fr: 1 µg/ml, WB: 1.0 - 2 µg/ml

Molecular weight

Approximately 40 kDa in HeLa cell lysates.

Protein Length

16

Imunogen

A peptide corresponding to 16 amino acids near the N-terminus of human Actin beta.

Specificitate

This antibody recognises human actin beta (ACTB), one of the cytoplasmic actin isoforms, a 41.7 kDa cytoskeletal protein. Actins are highly conserved proteins that are involved in cell motility, structure and integrity and are ubiquitously expressed in all eukaryotic cells.Higher vertebrates express six actin isoforms encoded for by distinct genes. Actin isoforms are highly evolutionarily conserved. Two striated muscle isoforms, alpha skeletal and alpha cardiac, two smooth muscle isoforms, alpha smooth and gamma smooth together with two cytoplasmic isoforms, beta cytoplasmic and gamma cytoplasmic are encoded by genes on chromosomes 1, 15, 10, 2, 7 and 17 respectively. All actin isoforms have highly homologous nucleotide sequences with the heterogeneity most notably in the N terminal region. However, small differences in primary structure here and elsewhere affect the conformational state of the various isoforms and their affinity for a range of actin binding proteins (ABPs). The range of affinities for ABPs suggests that where multiple globular monomeric (G-actin) forms exist at the same site, polymerisation may result in the formation of filamentous (F-actin) enriched for one particular isoform.Defects in actin beta are a cause of juvenile dystonia, a condition that results in movement disorders due to disordered muscle tonicity, abnormal posture, development malformations and sensory hearing loss.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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