Vitamin B12 ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărimi disponibile

48T · 96T

Țintă

Vitamin B12

Applications

Competitive ELISA

Reactivity

Universal

Assay type

Competitive (quantitative)

Sensitivity

3.80 pg/ml

Sample type

Serum, plasma, tissue homogenates or other biological fluids.

Detection type

Colourmetric

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

4.68-300 pg/ml

Assay Time

2h 30m

Principiul testului

Vitamin B12 ELISA Kit (A3003) employs the competitive enzyme immunoassay technique for the quantitative measurement of Vitamin B12 in serum, plasma, tissue homogenates or other biological fluids. The 96-well microtiter plate has been pre-coated with Vitamin B12 antigen. During the incubation, Vitamin B12 present in the samples or standards competes with the fixed amount of immobilized Vitamin B12 for binding sites on the Biotinylated Anti-Vitamin B12 Antibody. The more Vitamin B12 present in a sample or standard, the less Biotinylated Anti-Vitamin B12 Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Vitamin B12 Antibody is removed by washing, and an HRP-Avidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Vitamin B12 present in each sample or standard. The concentration of Vitamin B12 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Storage

Store kit components at +4°C or -20°C as instructed. Do not use past expiration date!

Documentație

• Manual de utilizare (PDF)se deschide pe site-ul producătorului