Universal hepatitis A virus Antibody ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

hepatitis A virus Antibody

Applications

Competitive ELISA

Reactivity

Universal

Assay type

Competitive (qualitative)

Sensitivity

Qualitative

Sample type

Serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.

Cross reactivity

Demonstrates specificity for hepatitis A virus antibodies with no reported cross-reactivity to related analogs.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

Qualitative

Assay Time

2 hours

Principiul testului

Universal hepatitis A virus Antibody ELISA Kit (A335466) is a 2 hour competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of universal hepatitis A virus Antibody in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids. A 96-well microtiter plate has been pre-coated with hepatitis A virus Antibody. During the assay, hepatitis A virus Antibody present in the standards or samples competes with immobilized hepatitis A virus Antibody for binding to an assay-specific antibody. Following incubation, unbound components are removed by washing and HRP-Streptavidin conjugate is added to the wells. After further incubation and washing, TMB substrate solution is added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the colour is measured at 450 nm using a microplate reader. The concentration of hepatitis A virus Antibody in the samples is inversely proportional to the OD450 value and is determined by comparison with the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.

Documentație

• Manual de utilizare (PDF)se deschide pe site-ul producătorului