Universal ACTH ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

ACTH

Applications

Sandwich ELISA

Reactivity

Universal

Recommended dilutions

Validated sample dilutions include Healthy human EDTA plasma (undiluted) and AtT-20 Cell Culture Supernatant (1:2).

Assay type

Sandwich (quantitative)

Sensitivity

7.5 pg/ml

Sample type

Plasma, cell culture supernatant, cell or tissue lysate, other liquid samples.

Cross reactivity

Specific for ACTH with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

12.5-800 pg/ml

Assay Time

2 hours

Cas number

CAS:53917-42-3

Principiul testului

Universal ACTH ELISA Kit (A337194) is a 2 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of universal ACTH in plasma, cell culture supernatant, cell or tissue lysate, other liquid samples. This QuickRun format uses a pre-coated 96-well microtiter plate and optimized ready-to-use reagents to reduce assay time. Standards and samples are added to the wells, where ACTH binds to the immobilized capture antibody. Assay-specific biotinylated detection antibody and HRP-Streptavidin reagents are then added according to the optimized QuickRun workflow. These reagents form an antibody-antigen-antibody sandwich complex with the captured ACTH. TMB substrate solution is added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of ACTH captured in each well. The concentration of ACTH is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.