Imagine indisponibilăUniversal 16,16-dimethyl Prostaglandin E2 ELISA Kit
Pe scurt
Universal 16,16-dimethyl Prostaglandin E2 ELISA Kit este un kit ELISA de tip Competitive (quantitative) pentru măsurarea 16,16-dimethyl Prostaglandin E2, cu reactivitate declarată pentru Universal. Măsoară în domeniul 31.25 - 2000 pg/ml, are o sensibilitate de 18.75 pg/ml, folosește detecție Colorimetric Detection (OD450) și se poate folosi pe probe de tip Serum, plasma, tissue homogenates, and other biological fluids. Se livrează în mărimea 96T, la prețul de 4.591,95 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Universal 16,16-dimethyl Prostaglandin E2 ELISA Kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of universal 16,16-dimethyl Prostaglandin E2 in serum, plasma, tissue homogenates, and other biological fluids.
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Specificații
- Brand
- Antibodies
- Mărime
- 96T
- Applications
- Competitive ELISA
- Reactivity
- Universal
- Assay type
- Competitive (quantitative)
- Sensitivity
- 18.75 pg/ml
- Cross reactivity
- Specific for 16,16-dimethyl Prostaglandin E2 with no reported cross-reactivity with other analogs or variants.
- Detection type
- Colorimetric Detection (OD450)
- Product range
- 31.25 - 2000 pg/ml
- Assay Time
- 2 hours
- Principiul testului
- Universal 16,16-dimethyl Prostaglandin E2 ELISA Kit (A319472) is a 2 hour competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of universal 16,16-dimethyl Prostaglandin E2 in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with 16,16-dimethyl Prostaglandin E2. During the assay, 16,16-dimethyl Prostaglandin E2 present in the standards or samples competes with immobilized 16,16-dimethyl Prostaglandin E2 for binding to an assay-specific antibody. Following incubation, unbound components are removed by washing and HRP-Streptavidin conjugate is added to the wells. After further incubation and washing, TMB substrate solution is added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the colour is measured at 450 nm using a microplate reader. The concentration of 16,16-dimethyl Prostaglandin E2 in the samples is inversely proportional to the OD450 value and is determined by comparison with the standard curve.
Documentație
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