Universal 1,25-Dihydroxy VitaminD3 ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

1,25-Dihydroxy Vitamin D3

Applications

Competitive ELISA

Reactivity

Universal

Assay type

Competitive (quantitative)

Sensitivity

3.75 pg/ml

Sample type

Serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.

Cross reactivity

Specific for 1,25-Dihydroxy Vitamin D3 with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

6.25-400 pg/ml

Assay Time

2 hours

Principiul testului

Universal 1,25-Dihydroxy Vitamin D3 ELISA Kit (A326312) is a 2 hour competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of universal 1,25-Dihydroxy Vitamin D3 in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids. A 96-well microtiter plate has been pre-coated with 1,25-Dihydroxy Vitamin D3. During the assay, 1,25-Dihydroxy Vitamin D3 present in the standards or samples competes with immobilized 1,25-Dihydroxy Vitamin D3 for binding to an assay-specific antibody. Following incubation, unbound components are removed by washing and HRP-Streptavidin conjugate is added to the wells. After further incubation and washing, TMB substrate solution is added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the colour is measured at 450 nm using a microplate reader. The concentration of 1,25-Dihydroxy Vitamin D3 in the samples is inversely proportional to the OD450 value and is determined by comparison with the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.

Documentație

• Manual de utilizare (PDF)se deschide pe site-ul producătorului