Species Independent His tag ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

His Tag

Applications

ELISA

Reactivity

Species independent

Formulation

Antibodies are supplied in Phosphate Buffered Saline with Tris-HCl.

Host

Rabbit

Product form

Recombinant protein is supplied lyophilized; antibodies and buffer are supplied in liquid form.

Assay type

Competitive (quantitative)

Sensitivity

4 ng/ml

Sample type

His-tagged proteins in mammalian and bacteria cell lysates or cell supernatant.

Detection type

Colorimetric

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

4 ng/ml -1000 ng/ml

Assay Time

1 hour

Imunogen

The immunogens for the antibodies in this kit are recombinant His-Tag.

Principiul testului

Species Independent His tag ELISA Kit (A334792) employs a one-step sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of species independent His tag in his-tagged proteins in mammalian and bacteria cell lysates or cell supernatant. A 96-well microtiter plate is provided pre-coated with an antibody specific for His tag. Standards and test samples are added to the wells, allowing His tag to bind to the immobilized capture antibody. A prepared antibody mixture is added and incubated to form antibody–antigen complexes. After incubation, unbound material is removed by washing, and TMB substrate is added to develop colour through an HRP-catalysed reaction. The reaction is stopped with acidic stop solution, producing a yellow endpoint signal. The intensity of the colour is proportional to the amount of His tag captured in each well. The concentration of His tag is determined by measuring absorbance at 450 nm and interpolating from the standard curve.

Storage

Shipped at +4 °C. For short-term storage, keep at +4 °C. Shelf life is 12 months from the date of receipt or reconstitution.