Imagine indisponibilăSheep Calprotectin ELISA Kit
Pe scurt
Sheep Calprotectin ELISA Kit este un kit ELISA de tip Sandwich (quantitative) pentru măsurarea Calprotectin, cu reactivitate declarată pentru Sheep. Măsoară în domeniul 1.563-100 ng/ml, are o sensibilitate de 0.938 ng/ml, folosește detecție Colorimetric Detection (OD450) și se poate folosi pe probe de tip Serum, plasma, tissue homogenates, and other biological fluids. Se livrează în mărimea 96T, la prețul de 5.091,08 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Sheep Calprotectin ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of sheep Calprotectin in serum, plasma, tissue homogenates, and other biological fluids.
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Specificații
- Brand
- Antibodies
- Mărime
- 96T
- Țintă
- Calprotectin
- Applications
- Sandwich ELISA
- Reactivity
- Sheep
- Assay type
- Sandwich (quantitative)
- Sensitivity
- 0.938 ng/ml
- Cross reactivity
- Specific for Calprotectin with no reported cross-reactivity with other analogs or variants.
- Detection type
- Colorimetric Detection (OD450)
- Product range
- 1.563-100 ng/ml
- Assay Time
- 4 hours
- Principiul testului
- Sheep Calprotectin ELISA Kit (A86978) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of sheep Calprotectin in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for Calprotectin. Standards and samples are added to the wells, where Calprotectin binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and a biotinylated detection antibody specific for Calprotectin is added to the wells. The detection antibody binds the captured Calprotectin to form an antibody-antigen-antibody sandwich complex. After further incubation and washing, HRP-Streptavidin conjugate is added to the wells. TMB substrate solution is then added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of Calprotectin captured in each well. The concentration of Calprotectin is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.
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