SARS-CoV-2 SARS-CoV-2 Spike Glycoprotein ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

SARS-CoV-2 Spike Glycoprotein

Applications

Sandwich ELISA

Reactivity

SARS-CoV-2 (Coronavirus, COVID-19, 2019-nCoV)

Recommended dilutions

Serum and plasma samples may affect test results and should be diluted at least 1:2 with Sample Dilution Buffer prior to testing. Validated sample dilutions include HEK293T cells (transfected for 1 day) (1:10) and Healthy serum (1:2).

Assay type

Sandwich (quantitative)

Sensitivity

390 fg/ml

Sample type

Plasma, tissue homogenates, and other biological fluids.

Cross reactivity

Specific for SARS-CoV-2 Spike Glycoprotein with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

0.78-50 pg/ml

Assay Time

5 hours

Principiul testului

SARS-CoV-2 Spike Glycoprotein ELISA Kit (High Sensitivity) (A302540) is a 5 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of coronavirus (covid-19, sars-cov-2, 2019-ncov) SARS-CoV-2 Spike Glycoprotein in plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for SARS-CoV-2 Spike Glycoprotein. Standards and samples are added to the wells, where SARS-CoV-2 Spike Glycoprotein binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and a biotinylated detection antibody specific for SARS-CoV-2 Spike Glycoprotein is added to the wells. The detection antibody binds the captured SARS-CoV-2 Spike Glycoprotein to form an antibody-antigen-antibody sandwich complex. After further incubation and washing, HRP-Streptavidin conjugate is added to the wells. TMB substrate solution is then added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of SARS-CoV-2 Spike Glycoprotein captured in each well. The concentration of SARS-CoV-2 Spike Glycoprotein is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.