Recombinant Tilapia Leptin B Protein

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Specificații

Brand

Antibodies

Mărimi disponibile

20µg · 100µg · 1mg

Țintă

Leptin B

Applications

SDS-PAGE

Formulation

Lyophilized from a solution containing Sodium Bicarbonate at 1:2 - salt:protein ratio.

Product form

Lyophilized

Concentration

1 mg/ml

Purity

>95% as determined by Gel Filtration and SDS-PAGE.

Reconstitution

Reconstitute with water or 0.4% Sodium Bicarbonate adjusted to pH 8-9, to no less than 0.1 mg/ml, which can then be further diluted to other aqueous solutions.

Expression system

Escherichia coli

Nature

Recombinant

Protein species

Tilapia

Protein Length

Full length protein.

Secvență

MRNILALLCVFLMAADQSTILLTKGESIKNTIHNIVNIAQITLVHIKKLKLPATPTEVPTPSIDGLSSISHDLGVLDNELQHPFLIQIQADVSSLEGRVRSFALSMECPLKPKPAVQTDESVFPDSRLYMTVAKVQHYLEKLILNKGKLKLC

Activitate biologică

Tilapia leptins were found to be biologically active in promoting proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor, but their activity was lower than that of mammalian leptin. Furthermore, the Tilapia leptins were biologically active in promoting STAT-LUC activation in COS7 cells transfected with Tilapia leptin receptor but not in cells transfected with human leptin receptor. Tilapia Leptin A was more active than Tilapia Leptin B.

Storage

Shipped at ambient temperature. Lyophilized: Short term (3 weeks) store at 25°C. Long term store desiccated below -18°C. Reconstituted: Short term (2-7 days) store at 4°C. Long term store below -18°C, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze/thaw cycles.

Documentație

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