Recombinant Rat Latent TGF-beta 1 Protein (N-terminal His Tag)

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Specificații

Brand

Antibodies

Mărimi disponibile

20µg · 50µg

Țintă

Latent TGF beta 1

Formulation

Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).

Conjugate

Unconjugated

Product form

Lyophilized

Concentration

Reconstitution dependent.

Purity

> 95% (by SDS-PAGE).

Reconstitution

Centrifuge the vial before opening. Reconstitute with sterile 4 mM HCl to a concentration of 0.1-0.5 mg/mL. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer.

Expression system

HEK293 cells

Nature

Recombinant

Protein species

Rat

Endotoxin level

<0.1 EU/µg of the protein by LAL method.

Etichetă (tag)

N-terminal His Tag

Secvență

LSTCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESADPEPEPEADYYAKEVTRVLMVDRNNAIYDKTKDITHSIYMFFNTSDIREAVPEPPLLSRAELRLQRFKSTVEQHVELYQKYSNNSWRYLGNRLLTPTDTPEWLSFDVTGVVRQWLNQGDGIQGFRFSAHCSCDSKDNVLHVEINGISPKRRGDLGTIHDMNRPFLLLMATPLERAQHLHSSRHRRALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASASPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS

Activitate biologică

Inhibition of cell proliferation of TF-1 human erythroleukemic cells: ED50 = 0.05-0.2ng/mL; Specific activity = 5.0×10^6-2.0×10^7units/mg. Stimulation of BMP7 expression in Rat fibroblast cells: 20ng/mL after 48h. Inhibition of the IL-4-dependent proliferation of HT-2 mouse T cells: ED50 = 0.78-3.1ng/mL; Specific activity = 3.23×10^5~1.28×10^6 units/mg.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.

Documentație

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