Recombinant Mouse TNFRSF1B Protein (C-terminal Human Fc Tag)

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Specificații

Brand

Antibodies

Mărimi disponibile

20µg · 50µg

Țintă

TNFRSF1B

Formulation

Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).

Conjugate

Unconjugated

Product form

Lyophilized

Concentration

Reconstitution dependent.

Purity

> 95% (by SDS-PAGE).

Reconstitution

Centrifuge the vial before opening and reconstitute with distilled sterile water to a concentration of 0.1-0.5 mg/mL. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer.

Expression system

HEK293 cells

Nature

Recombinant

Protein species

Mouse

Endotoxin level

<0.1 EU/µg.

Etichetă (tag)

C-terminal Human Fc Tag

Secvență

VPAQVVLTPYKPEPGYECQISQEYYDRKAQMCCAKCPPGQYVKHFCNKTSDTVCADCEASMYTQVWNQFRTCLSCSSSCTTDQVEIRACTKQQNRVCACEAGRYCALKTHSGSCRQCMRLSKCGPGFGVASSRAPNGNVLCKACAPGTFSDTTSSTDVCRPHRICSILAIPGNASTDAVCAPESPTLSAIPRTLYVSQPEPTRSQPLDQEPGPSQTPSILTSLGSTPIIEQSTKGG

Activitate biologică

Immobilized Mouse TNF-a (2 µg/mL) bind Mouse TNFRSF1B in a functional ELISA with a linear range of 0.02-0.77 ng/mL. Cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D: ED50 = 0.025-0.1 ng/mL in the presence of 0.1 ng/mL of Recombinant Mouse TNFa.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.

Documentație

• Fișă tehnică (PDF)se deschide pe site-ul producătorului