Recombinant Mouse IL-17A Protein (C-terminal His Tag)

Pe scurt

Specificații

Brand

Antibodies

Mărimi disponibile

20µg · 50µg

Țintă

IL-17A

Formulation

Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).

Conjugate

Unconjugated

Product form

Lyophilized

Concentration

Reconstitution dependent.

Purity

> 90% (by SDS-PAGE)

Reconstitution

Centrifuge the vial before opening and reconstitute with distilled sterile water to a concentration of 0.1-0.5 mg/mL. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer.

Expression system

HEK293 cells

Nature

Recombinant

Protein species

Mouse

Endotoxin level

<0.1 EU/µg.

Etichetă (tag)

C-terminal His Tag

Secvență

AAIIPQSSACPNTEAKDFLQNVKVNLKVFNSLGAKVSSRRPSDYLNRSTSPWTLHRNEDPDRYPSVIWEAQCRHQRCVNAEGKLDHHMNSVLIQQEILVLKREPESCPFTFRVEKMLVGVGCTCVASIVRQAA

Activitate biologică

Immobilized Mouse IL-17A/CTLA-8 (2 µg/mL) bind Mouse IL-17RA/CD217 in a functional ELISA with a linear range of 0.1-26 ng/mL. Induction of the IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells in the presense of 2 ng/mL TNFa: ED50 = 0.26-1.04 ng/mL; Specific activity =9.62×10^5~3.85×10^6 units/mg.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.

Documentație

• Fișă tehnică (PDF)se deschide pe site-ul producătorului