Recombinant Mouse IL-1 beta Protein

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Specificații

Brand

Antibodies

Mărime

10µg

Țintă

IL-1 beta

Applications

ELISA, WB, Functional Assays

Product form

Lyophilized

Concentration

100 µg/ml after reconstitution.

Recommended dilutions

ELISA: 0.2 - 0.4ng/well, WB: 1.5 - 3ng/lane

Purity

>98% (by SDS-PAGE and HPLC).

Reconstitution

Reconstitute with 0.1 ml of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution.

Expression system

Escherichia coli

Nature

Recombinant

Protein species

Mouse

Endotoxin level

< 0.1 ng/µg

Specificitate

Interleukin-1 beta (IL-1 beta), is a 17.5 kDa proinflammatory, cell-associated cytokine, produced by monocytes, tissue macrophages, keratinocytes and other epithelial cells. IL-1 beta promotes the proliferation of memory T cells and cytokine production. It attenuates regulatory T cell function and enables CD4+CD25- autoreactive effectors to avoid suppression. IL-1 beta signals downstream of myc and is required for angiogenesis and invasiveness of tumor cells. IL-1 beta is directly involved in neuronal injury in neurodegenerative disorders, and stimulates mitogenic FGF-like activity, bone resorption and promotes the release of collagenase and prostaglandin from synovial cells.

Storage

Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Documentație

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