Recombinant Human Zinc alpha 2 Glycoprotein

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Specificații

Brand

Antibodies

Mărimi disponibile

2µg · 10µg · 100µg

Țintă

Zinc alpha 2 Glycoprotein

Applications

SDS-PAGE

Formulation

Lyophilized from 100 mM Tris HCl Buffer, pH 8, with 150 mM Sodium Chloride.

Product form

Lyophilized

Concentration

0.5mg /ml

Purity

>90% as determined by RP-HPLC and SDS-PAGE.

Reconstitution

Reconstitute with deionized water to 0.5 mg/ml and let the lyophilized pellet dissolve completely.

Expression system

HEK293 cells

Nature

Recombinant

Protein species

Human

Protein Length

Protein fragment

Secvență

ASWSHPQFEK GSQENQDGRY SLTYIYTGLS KHVEDVPAFQ ALGSLNDLQF FRYNSKDRKS QPMGLWRQVEGMEDWKQDSQ LQKAREDIFM ETLKDIVEYY NDSNGSHVLQ GRFGCEIENN RSSGAFWKYY YDGKDYIEFNKEIPAWVPFD PAAQITKQKW EAEPVYVQRA KAYLEEECPA TLRKYLKYSK NILDRQDPPS VVVTSHQAPG EKKKLKCLAYDFYPGKIDVH WTRAGEVQEP ELRGDVLHNG NGTYQSWVVV AVPPQDTAPY SCHVQHSSLA QPLVVPWEAS

Activitate biologică

Differentiated human SGBS adipocytes were incubated for 18 h at two dose levels of rhZA2G - 5 and 20 µg/ml. Lipolysis was quantified by measuring glycerol release into the medium using a standard protocol. Isoproterenol (10 µM) and IBMX (100 µM) were used as positive controls. "Con" stands for the negative control. There was a 3-fold increase in glycerol release with both doses. The increase was statistically significant at 5 µg/ml dose of rhZA2G (p<0.01) as well as in positive controls.

Storage

Shipped at ambient temperature. Lyophilized: Short term (3 weeks) store at 25°C. Long term store desiccated below -18°C. Reconstituted: Short term (2-7 days) store at 4°C. Long term store below -18°C, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze/thaw cycles.

Documentație

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