Recombinant Human TWEAKR/FN14 Protein (C-terminal Human Fc and His Tag)

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Specificații

Brand

Antibodies

Mărime

50µg

Țintă

TWEAKR/FN14

Formulation

Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).

Conjugate

Unconjugated

Product form

Lyophilized

Concentration

Reconstitution dependent.

Purity

> 90% (by SDS-PAGE)

Reconstitution

Centrifuge the vial before opening and reconstitute with distilled sterile water to a concentration of 0.1-0.5 mg/mL. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer.

Expression system

HEK293 cells

Nature

Recombinant

Protein species

Human

Endotoxin level

<0.1 EU/µg of the protein by LAL method.

Etichetă (tag)

C-terminal Human Fc and His Tag

Secvență

EQAPGTAPCSRGSSWSADLDKCMDCASCRARPHSDFCLGCAAAPPAPFRLLW

Activitate biologică

Inhibition of the TWEAK-induced apoptosis in HT-29 human colon adenocarcinoma cells: ED50 = 2-12 µg/mL in the presence of 1 µg/mL recombinant human TWEAK. Immobilized Human TNFSF12 at 2 µg/mL bind Human TNFRSF12A in a functional ELISA with a linear range of 0.1-2.3 ng/mL. Inhibition of the the TWEAK-dependent proliferation of HUVEC human umbilical vein endothelial cells: ED50 = 20-80 ng/mL in the presence of 15 ng/mL recombinant human TWEAK.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.

Documentație

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