Recombinant Human TGF beta 1 Protein (N-terminal His Tag)

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Specificații

Brand

Antibodies

Mărimi disponibile

20µg · 50µg

Țintă

TGF beta 1

Formulation

Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).

Conjugate

Unconjugated

Product form

Lyophilized

Concentration

Reconstitution dependent.

Purity

> 95% (by SDS-PAGE).

Reconstitution

Centrifuge the vial before opening. Reconstitute with sterile 4 mM HCl to a concentration of 0.1-0.5 mg/mL. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer.

Expression system

HEK293 cells

Nature

Recombinant

Protein species

Human

Endotoxin level

<0.1 EU/µg of the protein by LAL method.

Etichetă (tag)

N-terminal His Tag

Secvență

LSTSKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESAEPEPEPEADYYAKEVTRVLMVETHNEIYDKFKQSTHSIYMFFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLSRGGEIEGFRLSAHCSCDSRDNTLQVDINGFTTGRRGDLATIHGMNRPFLLLMATPLERAQHLQSSRHRRALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASAAPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS

Activitate biologică

Inhibition of the cell proliferation of TF-1 human erythroleukemic cells: ED50 = typically 0.5-2 ng/mL; Specific activity =5.0×10^5~2.0×10^6 units/mg. Immobilized recombinant human TGFB1 at (2 µg/mL) bind recombinant human TGFBR2 in a functional ELISA with a linear range of 1-5 ng/mL. Inhibition of the the IL-4-dependent proliferation of HT-2 mouse T cells: ED50 = 9.75-39 ng/mL; Specific activity =2.6×10^4~1.0×10^5 units/mg.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.

Documentație

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