Recombinant Human SIRP alpha Protein (C-terminal Human Fc and His Tag)

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Specificații

Brand

Antibodies

Mărimi disponibile

10µg · 20µg

Țintă

SIRP alpha

Formulation

Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).

Conjugate

Unconjugated

Product form

Lyophilized

Concentration

Reconstitution dependent.

Purity

> 95% (by SDS-PAGE).

Reconstitution

Centrifuge the vial before opening and reconstitute with distilled sterile water to a concentration of 0.1-0.5 mg/mL. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer.

Expression system

HEK293 cells

Nature

Recombinant

Protein species

Human

Endotoxin level

<0.1 EU/µg of the protein by LAL method.

Etichetă (tag)

C-terminal Human Fc and His Tag

Secvență

EEELQVIQPDKSVLVAAGETATLRCTATSLIPVGPIQWFRGAGPGRELIYNQKEGHFPRVTTVSDLTKRNNMDFSIRIGNITPADAGTYYCVKFRKGSPDDVEFKSGAGTELSVRAKPSAPVVSGPAARATPQHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPVGESVSYSIHSTAKVVLTREDVHSQVICEVAHVTLQGDPLRGTANLSETIRVPPTLEVTQQPVRAENQVNVTCQVRKFYPQRLQLTWLENGNVSRTETASTVTENKDGTYNWMSWLLVNVSAHRDDVKLTCQVEHDGQPAVSKSHDLKVSAHPKEQGSNTAAENTGSNER

Activitate biologică

Immobilized Recombinant Human CD47 (1 µg/mL) bind Recombinant Human SIRP alpha in a functional ELISA with a linear range of 1-6 ng/mL. Loaded Human SIRP-alpha/CD172a Protein, C-terminal Human Fc and Hison Tag, ProA Biosensor, can bind Human CD47 Protein, C-terminal His Tag, in BLI assay (Gator) with an affinity constant of 11.1 nM. Immobilized protein to support the adhesion of the OVCAR-3 cells: ED50 = 0.53-2.12 µg/mL.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.

Documentație

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