Recombinant Human Resistin Protein

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Specificații

Brand

Antibodies

Mărime

25µg

Țintă

Resistin

Applications

ELISA, WB, Functional Assays

Formulation

Supplied in 10mM Sodium Citrate.

Product form

Lyophilized

Concentration

100 µg/ml after reconstitution.

Recommended dilutions

ELISA: 0.2 - 0.4ng/well, WB: 1.5 - 3ng/lane

Purity

>98% (by SDS-PAGE and HPLC).

Reconstitution

Reconstitute with 250 µl of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution.

Expression system

Escherichia coli

Nature

Recombinant

Protein species

Human

Endotoxin level

< 1 EU/µg

Specificitate

Human resistin, a disulfide-linked homodimeric protein otherwise known as adipose tissue-specific secretory factor, is an adipose-derived hormone and member of the resistin/FIZZ family, implicated as a suppressor of the ability of insulin to reduce blood sugar, and to play a role in type 2 diabetes and obesity.Resistin is involved in an increase in the transcription/expression of many pro-inflammatory cytokines, including interleukin-1,-6 and -12, and also upregulates vascular cell-adhesion molecule-1 (VCAM-1), intracellular adhesion molecule-1 (ICAM-1), and monocyte chemotactic protein-1 (MCP-1/CCL2).

Storage

Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Documentație

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