Recombinant Human MMP3 Protein (C-terminal His Tag)

Pe scurt

Specificații

Brand

Antibodies

Mărimi disponibile

2µg · 10µg · 1mg

Țintă

MMP3

Applications

SDS-PAGE

Formulation

Supplied in 20 mM Tris HCl Buffer, pH 7.5, with 150 mM Sodium Chloride and 0.05% Brij 35.

Product form

Liquid

Concentration

Lot Specific

Purity

>95% as determined by SDS-PAGE.

Expression system

HEK293 cells

Nature

Recombinant

Protein species

Human

Etichetă (tag)

C-terminal His Tag

Activitate biologică

The activity was measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The specific activity is >150 pmoles/min/µg.Recombinant Human MMP-3 protein pro form needs to be activated with Chymotrypsin.Activation Protocol:1. Dilute MMP3 to 20µg/ml in the Assay Buffer: 50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% (w/v) and Brij 35, pH 7.5.2. Activate MMP3 by adding Chymotrypsin(Sigma, Catalog#C­3142,1mg/ml stock in 1mM HCl) to a final concentration of 5µg/ml.3. Incubate at 37°C for 30 minutes.4. Stop activation with 2mM PMSF. Pre-warm the PMSF to 37°C prior to adding to sample.

Storage

Shipped at 4°C. Short term (2-4 weeks) store at 4°C. Long term store at -20°C. Avoid freeze/thaw cycles.

Documentație

• Fișă tehnică (PDF)se deschide pe site-ul producătorului