Recombinant Human MMP1 Protein (C-terminal His Tag)

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Specificații

Brand

Antibodies

Mărimi disponibile

2µg · 10µg · 1mg

Țintă

MMP1

Applications

SDS-PAGE

Formulation

Supplied in MES Buffer with Sodium Chloride, Brij35, and Glycerol.

Product form

Liquid

Concentration

Lot Specific

Purity

>95% as determined by SDS-PAGE.

Expression system

HEK293 cells

Nature

Recombinant

Protein species

Human

Etichetă (tag)

C-terminal His Tag

Activitate biologică

The activity was measured by its ability to cleave fluorogenic peptide substrate, Mca-KPLGL-Dpa-AR-NH2, The specific activity is >400 pmoles/min/µg.Recombinant Human MMP-1 protein pro form needs to be activated with p-aminophenylmercuric acetate (APMA).Activation Protocol:1. Dilute MMP1 to 50µg/ml in the Assay Buffer: 50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% (w/v) and Brij 35, pH 7.5.2. Activate MMP1 by adding APMA to a final concentration of 1mM. (Sigma, Catalog # A9563) and 100mM stock in DMSO.3. Incubate at 37°C for 2 hours.

Storage

Shipped at 4°C. Short term (2-4 weeks) store at 4°C. Long term store at -20°C. Avoid freeze/thaw cycles.

Documentație

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