Recombinant Human MCP1 Protein

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Specificații

Brand

Antibodies

Mărime

20µg

Țintă

MCP1

Applications

ELISA, WB, Functional Assays

Product form

Lyophilized

Concentration

100 µg/ml after reconstitution.

Recommended dilutions

ELISA: 0.2 - 0.4ng/well, Functional Assays: 10ng/ml - 100ng/ml, WB: 1.5 - 3ng/lane

Purity

>98% (by SDS-PAGE and HPLC).

Reconstitution

Reconstitute with 0.2 ml of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution.

Expression system

Escherichia coli

Nature

Recombinant

Protein species

Human

Endotoxin level

< 0.1 ng/µg

Specificitate

Recombinant human MCP-1/MCAF also known as C-C motif chemokine 2, Monocyte chemoattractant protein 1, Monocyte chemoattractant protein 1, HC11 or Small-inducible cytokine A2 is encoded by the CCL2 gene. Recombinant MCP-1 is a 76 amino acid ~13kDa chemokine involved in chemotaxis and polarization of the immune response.Recombinant human MCP-1/MCAF has been employed successfully as a control antigen in the development of a sensitive ELISA employing Mouse anti Human MCP-1 antibody clone 2.2-4A4-1A11 as a capture reagent and biotinylated Rabbit anti Human MCP-1 polyclonal antibody (AHP784B) as a detection reagent.

Storage

Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Documentație

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