Recombinant Human Leptin Protein

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Specificații

Brand

Antibodies

Mărime

1mg

Țintă

Leptin

Applications

ELISA, WB, Functional Assays

Formulation

Supplied with 0.1% TFA.

Product form

Lyophilized

Concentration

1 mg/ml after reconstitution.

Purification

0.2µM sterile filtered.

Recommended dilutions

ELISA: 0.2 - 0.4ng/well, WB: 1.5 - 3ng/lane

Purity

>98% (by SDS-PAGE and HPLC).

Reconstitution

Reconstitute with 1 ml of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution. For long term storage the addition of 0.1% Bovine Serum Abumin is recommended.

Expression system

Escherichia coli

Nature

Recombinant

Protein species

Human

Endotoxin level

< 1 EU/µg

Specificitate

Recombinant human leptin is a leptin preparation made in E. coli. Leptin is a major adipose-derived hormone and member of the leptin family, which plays a key role in appetite control and the regulation of energy expenditure.Secreted leptin associates with proteins within the bloodstream and is transported to the brain, where it inhibits or up-regulates the activity of neuropeptides such as neuropeptide Y (NPY) and alpha-melanocyte-stimulating hormone respectively. Leptin is a recognised marker of obesity, with a correlation between circulating levels of leptin and body fat mass, and defects in leptin production result in severe obesity. Regulators of leptin expression include insulin, cortisol, cAMP and thiazolidinediones.

Storage

Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Documentație

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