Recombinant Human IL-7 Protein (C-terminal His Tag)

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Specificații

Brand

Antibodies

Mărimi disponibile

20µg · 50µg

Țintă

IL-7

Formulation

Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).

Conjugate

Unconjugated

Product form

Lyophilized

Concentration

Reconstitution dependent.

Purity

> 90% (by SDS-PAGE)

Reconstitution

Centrifuge the vial before opening and reconstitute with distilled sterile water to a concentration of 0.1-0.5 mg/mL. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer.

Expression system

HEK293 cells

Nature

Recombinant

Protein species

Human

Endotoxin level

<0.1 EU/µg of the protein by LAL method.

Etichetă (tag)

C-terminal His Tag

Secvență

DCDIEGKDGKQYESVLMVSIDQLLDSMKEIGSNCLNNEFNFFKRHICDANKEGMFLFRAARKLRQFLKMNSTGDFDLHLLKVSEGTTILLNCTGQVKGRKPAALGEAQPTKSLEENKSLKEQKKLNDLCFLKRLLQEIKTCWNKILMGTKEH

Activitate biologică

Immobilized Human IL7 (2 µg/mL) bind human IL7R in a functional ELISA with a linear range of 0.97 -159.1 ng/mL. Cell proliferation assay using IL-2-activated mouse spleen cell: ED50 = 0.1-0.4 ng/mL; Specific activity =2.5×10^6~1×10^7 units/mg. Cell proliferation assay using murine 2E8 cells: ED50 = 0.78-3.1ng/mL; Specific activity =3.23×10^5~1.28×10^6 units/mg.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.

Documentație

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