Recombinant Human IL-15 Protein (C-terminal Human Fc and His Tag)

Pe scurt

Specificații

Brand

Antibodies

Mărime

20µg

Țintă

IL-15

Formulation

Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).

Conjugate

Unconjugated

Product form

Lyophilized

Concentration

Reconstitution dependent.

Purity

> 95% (by SDS-PAGE).

Reconstitution

Centrifuge the vial before opening and reconstitute with distilled sterile water to a concentration of 0.1-0.5 mg/mL. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer.

Expression system

HEK293 cells

Nature

Recombinant

Protein species

Human

Endotoxin level

<0.1 EU/µg of the protein by LAL method.

Etichetă (tag)

C-terminal Human Fc and His Tag

Secvență

NWVNVISDLKKIEDLIQSMHIDATLYTESDVHPSCKVTAMKCFLLELQVISLESGDASIHDTVENLIILANNSLSSNGNVTESGCKECEELEEKNIKEFLQSFVHIVQMFINTS

Activitate biologică

Inhibition of cell proliferation for HT-2 cells: ED50 = 2-8ng/mL; Specific activity = 1.25 × 10^5~5.0× 10^5 units/mg. Inhibition of cell proliferation for MO7e human megakaryocytic leukemic cells: ED50 = 24.29-97.14ng/mL; Specific activity = 1.03 × 10^4~4.12× 10^4 units/mg.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.

Documentație

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