Recombinant Human IL-12 Protein

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Specificații

Brand

Antibodies

Mărime

10µg

Țintă

IL-12

Applications

ELISA, Functional Assays, WB

Formulation

Supplied in Phosphate Buffered Saline.

Product form

Lyophilized

Concentration

200 µg/ml after reconstitution.

Recommended dilutions

ELISA: 0.2 - 0.4 ng/well, Functional Assays: 0.1 - 10 ng/ml, WB: 1.5 - 3.0 ng/lane

Purity

>98% (by SDS-PAGE and HPLC).

Reconstitution

Reconstitute with 50 µl sterile Phosphate Buffered Saline. For long term storage the addition of 0.1% Bovine Serum Albumin is recommended. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution. Do not vortex.

Expression system

CHO

Nature

Recombinant

Protein species

Human

Endotoxin level

< 0.1 ng/µg

Specificitate

Recombinant human Interleukin-12 (IL-12) produced in CHO cells is a ~75 kDa glycoprotein comprised of disulfide-linked 35kDa (p35) and 40kDa (p40) subunits.IL-12 is produced by activated B lymphocytes and macrophages. The properties of IL-12 include the stimulation of growth of activated T lymphocytes, and actions in the development of Th1 CD4 +ve cells and CD8 +ve cytotoxic T cells. IL-12 also promotes activity and division of NK cells and promotes formation of TH1 cells producing &gamma-interferon.

Storage

Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Documentație

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