Recombinant Human IL-11 Protein

Pe scurt

Specificații

Brand

Antibodies

Mărime

10µg

Țintă

IL-11

Applications

ELISA

Formulation

Supplied in 50 mM TRIS-HCl, pH 8, with 0.15 M NaCl, 1% Trehalose, and 0.05% Tween 80.

Product form

Lyophilized

Concentration

100 µg/ml after reconstitution.

Purification

Affinity-chromatography

Purity

=95% (by coomassie blue staining of a reducing SDS-PAGE gel).

Reconstitution

Centrifuge vial prior to reconstitution. Reconstitute to 100 µg/ml by adding 100 µl double-distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution. Do not vortex.

Expression system

Escherichia coli

Nature

Recombinant

Protein species

Human

Specificitate

Recombinant Human interleukin-11Interleukin-11 (IL-11), also known as AGIF belongs to the IL-6 family of cytokines, and plays a protective role in acute inflammatory settings (Traber et al. 2019). IL-11 has both pro- and anti-inflammatory functions through activation of signaling cascades (Lokau et al. 2017). IL-11 is largely absent in the body fluids of healthy people, but is upregulated in a series of diseases. IL-11 can be expressed in fibroblasts, and its inhibition has been suggested as a treatment for fibrotic disease (Schafer et al. 2017), including fibrotic cardiovascular diseases (Ye et al. 2019). A role of IL-11 has also been identified in development of inflammation-associated cancers, and its inhibition may be a therapeutic goal (Putoczki et al. 2013). This recombinant Human interleukin-11 can be used to create a standard curve in a sandwich ELISA with purified Mouse anti Human interleukin-11 antibody, clone D08-4C12 (MCA6204GA) as a capture antibody and biotinylated Mouse anti Human interleukin-11 antibody, clone C16-4B8 (MCA6205B) as the detection antibody.

Storage

Lyophilized: Store at -70°C. Reconstituted: Store undiluted at -80°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Documentație

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