Recombinant Human IL-1 beta Protein (C-terminal His Tag)

Pe scurt

Specificații

Brand

Antibodies

Mărimi disponibile

20µg · 50µg

Țintă

IL-1 beta

Formulation

Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).

Conjugate

Unconjugated

Product form

Lyophilized

Concentration

Reconstitution dependent.

Purity

> 95% (by SDS-PAGE).

Reconstitution

Centrifuge the vial before opening and reconstitute with distilled sterile water to a concentration of 0.1-0.5 mg/mL. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer.

Expression system

Escherichia coli

Nature

Recombinant

Protein species

Human

Endotoxin level

<0.1 EU/µg of the protein by LAL method.

Etichetă (tag)

C-terminal His Tag

Secvență

APVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS

Activitate biologică

NF-?B reporter assay in HEK293 cells: ED50 = 1-10 pg/mL; Specific activity = 1×10^8~1×10^9 units/mg. Cell proliferation assay using D10.G4.1 mouse helper T cells: ED50 = 0.217-0.870ng/mL; Specific activity = 1.15×10^6~4.61×10^6 units/mg. Immobilized Human IL-1 beta (10µg/mL) bind Biotinylated Human IL-1R1 in a functional ELISA with a linear range of 0.002-1µg/mL.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.

Documentație

• Fișă tehnică (PDF)se deschide pe site-ul producătorului