Recombinant Human GM-CSF Protein

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Specificații

Brand

Antibodies

Mărime

20µg

Țintă

GM-CSF

Applications

ELISA, WB, Functional Assays

Formulation

Supplied in 10mM Sodium Citrate, pH 3.5.

Product form

Lyophilized

Concentration

100 µg/ml after reconstitution.

Recommended dilutions

ELISA: 0.2 - 0.4ng/well, Functional Assays: 1ng/ml, WB: 1.5 - 3ng/lane

Purity

>98% (by SDS-PAGE and HPLC).

Reconstitution

Reconstitute with 0.2 ml of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution. For long term storage the addition of 0.1% Bovine Serum Albumin is recommended.

Expression system

Escherichia coli

Nature

Recombinant

Protein species

Human

Endotoxin level

< 1 EU/µg

Specificitate

Recombinant Human granulocyte-macrophage colony stimulating factoris a highly purified preparation of E. coli produced human GM-CSF.GM-CSF (granulocyte-macrophage colony-stimulating factor) is a haematopoietic growth factor which exists in both glycosylated and non-glycosylated biologically active forms, and stimulates the development of granulocytes, macrophages, early megakaryocytes and eosinophil progenitor cells. The ability of recombinant GM-CSF to increase haematopoietic cell recovery has become a focus area in the therapeutic treatment of patients following bone marrow transplantation.

Storage

Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Documentație

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