Recombinant Human Galectin 9 Protein (N-terminal His Tag)

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Specificații

Brand

Antibodies

Mărime

50µg

Țintă

Galectin-9

Formulation

Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).

Conjugate

Unconjugated

Product form

Lyophilized

Concentration

Reconstitution dependent.

Purity

>92% (by SDS-PAGE).

Reconstitution

Centrifuge the vial before opening and reconstitute with distilled sterile water to a concentration of 0.1-0.5 mg/mL. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer.

Expression system

HEK293 cells

Nature

Recombinant

Protein species

Human

Etichetă (tag)

N-terminal His Tag

Secvență

AFSGSQAPYLSPAVPFSGTIQGGLQDGLQITVNGTVLSSSGTRFAVNFQTGFSGNDIAFHFNPRFEDGGYVVCNTRQNGSWGPEERKTHMPFQKGMPFDLCFLVQSSDFKVMVNGILFVQYFHRVPFHRVDTISVNGSVQLSYISFQPPGVWPANPAPITQTVIHTVQSAPGQMFSTPAIPPMMYPHPAYPMPFITTILGGLYPSKSILLSGTVLPSAQRFHINLCSGNHIAFHLNPRFDENAVVRNTQIDNSWGSEERSLPRKMPFVRGQSFSVWILCEAHCLKVAVDGQHLFEYYHRLRNLPTINRLEVGGDIQLTHVQT

Activitate biologică

Immobilized Recombinant Human Galectin-9 (2 µg/mL can bind Recombinant Human TIM-3 in a functional ELISA with a linear range of 5-15 µg/mL. Immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells (8×10^4 cells/well) when added to LGALS9 coated plates (5 µg/mL and 100 µL/well) in the presence of 10µg/ml PHA: approximately 70-80% cells will adhere specifically after 60 minutes (37°C).

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.

Documentație

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